Monitoring of Leptospira in captive turtles by DNA analysis / [Monitoramento de Leptospira em quelônios mantidos em cativeiro pela análise de DNA]

Existem poucos estudos sobre doenças infecciosas em animais silvestres. O objetivo deste estudo foi pesquisar DNA de Leptospira spp. em sangue de tartarugas mantidas em cativeiro, pertencentes ao Bosque Rodrigues Alves (Jardim Zoobotânico da Amazônia). O DNA foi isolado das amostras de sangue coletadas de 148 tartarugas pertencentes a seis espécies diferentes. A reação em cadeia da polimerase (PCR) foi realizada utilizando-se iniciadores específicos para DNA de Leptospira spp. Nenhuma das amostras apresentou resultado positivo para Leptospira spp.(AU)

Productive performance and surface temperatures of Japanese quail exposed to different environment conditions at start of lay.

The objective of this study was to evaluate the effects of different environment conditions on productive performance and surface temperatures of Japanese quail (Coturnix coturnix japonica) during the initial stage of laying. In environmental controlled chambers, the birds were subjected to different temperatures and air velocities at the feeder. A total of 216 Japanese quails were distributed randomly in 2 galvanized wire cages, with 3 partitions each and 27 birds/cage. The experimental design consisted of randomized blocks with 2 treatments (air velocity at the feeder: 0, 1, 2, and 3 m/s and air temperature: 17, 23, 29, and 35°C) and 6 replicates. The productive performance was analyzed statistically (Sigma Plot 12.0) by 2-way ANOVA, with treatment means separated by the Tukey test (P < 0.05). To evaluate the main effects and interactions of the factors, the Holm-Sidak multiple comparisons test was performed using a mild condition as the control group (0 m/s). Feed intake did not differ (P > 0.05) among birds reared at temperatures of 23, 29, and 35°C, but higher feed intake was noted at 17°C. The mean values of egg production increased significantly (P < 0.05) with increased air velocity levels. It was observed that there was an increase in egg production and feed intake with the intensification of air velocity at the feeder, regardless of ambient temperature. Egg weight and feed conversion were not affected by air velocity treatments (P > 0.05). There was a significant positive correlation between air temperature and mean surface temperature and head surface temperature. In contrast, a significant negative correlation was observed between air velocity and mean surface temperature and head surface temperature. Productive performance was affected by temperature and air velocity, except for egg weight and feed conversion, which was not influenced by air velocity. Air velocity is important in removing heat from the surface of birds.

Can we document the practice of dispensing? A systematic review.

WHAT IS KNOWN: Documentation is a process indicator utilized to evaluate quality clinical pharmacist services. In this framework, documentation of dispensing, besides supporting patient care, allows evaluation of the impact of counselling. OBJECTIVE: To identify and assess studies of documentation by pharmacists in the dispensing process. METHOD: A systematic review was carried out according to the following steps: (i) identification of studies in the following databases: PubMed/Medline, Web of Science, Scopus and Lilacs, using the descriptors 'counselling', 'dispensing', 'community pharmacy services', 'pharmacies' and 'pharmacists' in different combinations; (ii) evaluation of studies, in which the title, abstract and full text of the studies, and the evaluation of the methodological quality of the selected studies were analysed. RESULTS: In total, 26 articles met the specific criteria. Few studies addressed documentation as part of the dispensing working process (3), and 16 articles reported documentation using non-electronic systems. The main variables documented were patient information, drug therapy problems and clinical interventions. Furthermore, 20 studies showed the effects of documentation in the dispensing process and 23 studies included statistical evidence. Eight articles met between 42% and 75% of the 28 items recommended by the STROBE initiative, and two articles met approximately 60% of the 36 items recommended by the CONSORT initiative. WHAT IS NEW AND CONCLUSION: Few studies address documentation as part of the dispensing working process. Thus, researchers should be concerned with standardizing documentation and implementing more robust designs and multicenter studies.